![]() ![]() Gene sequencing is the process that determines the nucleotide sequence of a particular gene or the whole genome.ĭNA fingerprinting involves a technique where DNA samples of two or more subjects are fragmented and analyzed to determine the identity of a person. What is the Difference Between Gene Sequencing and DNA Fingerprinting? Electrophoresis is used to determine the results in both techniques.In both cases, the sample analyzed is a DNA sample.What are the Similarities Between Gene Sequencing and DNA Fingerprinting? DNA is extracted from these available samples (hair/semen/spit/blood) and analyzed with the DNA samples of the suspects to determine the true culprit. Thus, the identity of a person could be confirmed through this technique.ĭNA fingerprinting is most often used in finding the true suspect of a crime scene by analyzing the available biological samples at the crime scene. If it is not similar, the gel images will not be identical. If the two samples are similar, the electrophoresis gel image should be identical for both samples. When two samples are brought for analysis, both samples are digested with the same restriction enzymes to yield fragments. This technique uses restriction enzymes, which are enzymes capable of chopping DNA at specific sequences. At present, DNA fingerprinting is done using the technique RFLP. ![]() In early days, DNA fingerprinting was done using a hybridization technique using fluorescent or radio labeled markers. It is also applied in the field of agricultural biotechnology to determine new varieties of plant species and to identify plant genes responsible for beneficial agronomic traits such as pest resistance, disease resistance, and drought resistance.ĭNA fingerprinting is a technique mainly used in forensic studies to confirm the identity of a person involved in a forensic investigation. Gene sequencing is mainly used to determine the sequence of a novel gene or to analyze mutations of a gene present in diseased states and to confirm the genetic basis of diseases.
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